When we get complete sequence of DNA (is it after last step of elongation in thermalcycler) when PCR ends.?

When we get complete sequence of DNA (is it after last step of elongation in thermalcycler) when PCR ends.

My 91 Acura legend dashbord keeps flashing when I put in D4 drive, is this something I should be worried about?

as this just started happening about a week ago. Also I notice at the same time my speedometer goes haywire occionally, is this all connected and is it something to concern about?

I don't even know this guy's name? Help!?

Well I went to Tennessee for my cousin's graduation and I live in Louisiana. Well we stayed at a hotel call 'Emby Suites' and it was very nice. Well we just got home from dinner with my cousin and the rest of the family and it was the night before his graduation. My family and I decided we would go swimming. Well when we got down to the pool area there were guys. Of course I just stayed in the hot tub. Well this one guy came over and got in. I found him attractive, as I do a lot of guys, but there was some different about him that made my stomach just jump into my throat. I of course got out after he left. And we headed back to our suite. This particular hotel was kind of square shaped and the rooms were on the sides so you could look over the railing outside your room and see what was going on in the lobby. Tired of my family's racket I stepped outside and looked over the railing (as there was a wedding dinner rehearsal that night) and just sitting there when I noticed the SAME guy from the hot tub walked out and was looking over the railing two floors down from me. I watched him from the corner of my and saw he periodically looked up at me. (I pretended I didn't notice) Finally he asked me if I was "Just chilling" and my whole stomach jumped. I was like "Yeah nothing better to do round here..." After that my mom came out and told me to come inside. The next morning I got up got dressed and headed down to the gift shop and I noticed he was down in the lobby too. I played it cool and pretended not to notice him. I walked in and saw him walk into the gift shop as well. I started looking around and realized I couldn't even afford a coffee mug! So I left and as I did I noticed he walked out to. As I walked into the elevator and punched the on he walked in... And he got on his floor and I went back to my room. Couple hours later I was getting ready for the graduation. My family was already downstairs waiting for me. And I had my cutest dress on. He watched me walk to the elevator and watched me as the elevator went down (they were gl so you could see who was in them) not trying to hide that he was clearly staring at me. I was soooo flattered. Then about 5 hours later we came back and as we walked to the front door I noticed he, his dad, and his friends were sitting on the couches next to the door. Well as I walked in he immediately stood up. I know WTF? Anyways, I realized I had major feelings for this guy yet I don't know his name. I knew the next morning we were leaving and I was really upset. Well morning came and we were carrying our bags out and I saw his family and his friend's family with their stuff piled up too. We kind of exchanged sad glances. And as we got down to the lobby (him, his family, his friend, and his friend's family were still on their floor) he was watching me and his friend was rubbing his back as if he was comforting him. I almost bursted into tears right there. Well as we started our nine hour journey home (which was today) I thought of him the WHOLE way. As soon as I got into my room I started bawling! Then I picked at myself saying that I'm pathetic crying over a guy that I'm basically strangers to and will most likely NEVER see again. I'm crying my eyes out right now because I know I'll never see him again. I have never known this feeling of my heart aching for him ad my stomach queasy all the time. Especially towards a guy whose name I don't even know! And I know you are probably thinking I'm just a lovesick teen but that isn't it. I've never felt this way, and I've never wanted someone so bad in my life. What is the feeling?!

Is my life ruined because i didnt get into a good college?

I applied to college and only got into 1 out of the many that i applied to. the one that i got into is mcgill university. Is that even a good school? my parents are not happy because they never heard of it. The only reason i applied there was because it was a backup school for me.

I need photo shoot ideas?

I have a s1000 fujifilm (FinePix). I have a photo shoot this may (2011) with a group of high school senors. The photo shoot will be in West Chester and/or downing town, Pennsylvania. I need some really FUN!! but also grown up, ideas. where should i do it at..ect? i usually know but i have photographer block! :(

Should i tell the headmaster or not?

Today I was waiting outside of school with my mate eve. we was waiting for my other two friends lizzie and aimee. when they finally came lizzie looked and me and said common then started to walk really fast so i shouted to her "why do you always do this. This is why we always fall out because you leave me" as soon as i said that an 70 year old man and woman went by and gave me an evil glance and said something but i didnt quite hear them. So i kindly went up to them saying sorry but wat did say (not in a mean way) then they said you could wash your dirty little mouth out and i said why then they said that i swore and me and eve plus lizzie and aimee said i didnt say that then they started saying lots of things to me and for the record im 14 and never had a detension before and i really nice person. anyway bak to the story they started to say stuff to my friends then they slowly walked for about 3 seconds when my next door neighbour said something im not quite sure wat then all of a sudden the old man went up to him and grabbed his neck and said you shouldnt say that. he was been really threatning and dont forget we are only 14 then his wife said hes not worth it and we all said let go off him. so he did then he walked off and we got huddled into a big crowed walkin home. About 3 mins afterwords we catched up to them and i said excuse me but i dont think wat you did to that boy was right. then he said wat did he do and me and my four friends said you grabbed the boys neck and he said he didnt but about 20 people saw him do it. then he wife started sayin that she is glad her grandkids arnt like me and stuff like that then me and my mate eve said sorry but i really didnt swear you must of herd me wrong then the old man said do you want me to do the same to you as i did to that boy. then the lady said give me your name im reporting you to the non-emergency police and school because you swore even tho i didnt. then i said im not givin my name cause i dint do anything your the people in the wrong. then he just went right up close to me and said leave it and i said ok. then they walked off. wen i got home i was in tears ive never been treatend before. my mates said they will go with me to the head and tell him wat happend because at our school they take thiss very seriously. but im a bit to scared to cause what if the police get involved i dont want to get in truoble for something i didnt do. even tho i didnt do anything. i told my mum and she said i should tell him. What would you do please help!

Why do some Christians take the Bible as 100% literal?

I hate people who cherry pick the bible for the good bits - when they do that they are essentially dictating what is right and wrong.